Screening for significant medium components for Pneumoncandin B0 production at shake flask level through factorial design

  • Umesh Luthra Ipca Laboratories Ltd., Biotech R&D, Kandivali (W), Mumbai- 400067, Maharashtra, India
  • Aditi Trivedi Ipca Laboratories Ltd., Biotech R&D, Kandivali (W), Mumbai- 400067, Maharashtra, India
  • Sneha Khadpekar Ipca Laboratories Ltd., Biotech R&D, Kandivali (W), Mumbai- 400067, Maharashtra, India
Keywords: Analysis of variance, full factorial design, media optimization, pneumocandin B0


Pneumocandin B0 is an antifungal agent which has broad spectrum activity. It is produced as a result of secondary metabolite production by fungus Zalerion arboricola. Efforts have been taken to enhance the yield by improving the process conditions and media optimization. The study uses a statistical approach for designing the media and studying the impact of each component on the yield.


Schwartz R.E.; Giacobbe R.A.; Bland J.A.; Monaghan R.L., A new antifungal agent. I. Fermentation and isolation, J. Antibiot. 42, 163–167 (1989)

Frosco M, Barrett JF, Importance of antifungal drug-resistance: clinical significance and need for novel therapy, Expert. Opin. Investig. Drugs. 7(2),175-198 (1998).

D. Weuster-Botz, Experimental design for fermentation media development: Statistical design or global random search, J. Biosci. Bioeng. 90, 473–483 (2000).

M.C.T. Damaso, C.M.M.C. Andrade, N. Pereira Jr., Production and properties of the cellulase-free xylanase from Thermomyces lanuginosus IOC-4145, Braz. J. Microbiol. 33, 333–338 (2002).

Zhang X., Li N., Li X., Lin Y., Zhang J., Lin Y., Jiang Q., Simultaneous determination of content of pneumocandin A0 and B0 in fermentation broth by HPLC,Huaxue Yu Shengwu Gongcheng. 28(8), 88-90 (2001).

Schwartz, Robert E., White, Raymond F., Liesch, Jerrold M., Hensens, Otto D., Joshua, Henry, Schmatz, Dennis M., Jkacz, Jan S., Kaplan, Louis, Sosa, Margaret S., Manufacture of a fungicidal echinocandin derivative with Zalerion arboricola. Eur. Pat. Appl. , EP 405997, (1991).

Connors N., Pollard D., Pneumocandin B0 production by fermentation of the fungus Glarea lozoyensis: physiological and engineering factors affecting titer and structural analogue formation, Mycology series. 22, 515-538 (2005).

Peterson L.A., Hughes D.L., Huges R., DiMichele L., Salmon P., Connors N,. Effects of aminoacid and trace element supplementation on pneumocandin production by Glarea lozoyensis: impact on titer, analogue levels and the identification of new analogues of pneumocandin B0, J. Ind. Microbiol. Biotechnol.26(4), 216-221 (2001)

Anderson M.J. and Whitcomb P.J., DOE Simplified: Practical Tools for Effective Experimentation., 2nd Ed. Productivity Press, NewYork, ISBN-10: 1563273446, 241 (2007).

.Manikan V, Mohd Sahaid M. K, Mohd Hafez Mohd H. M, Improved prediction for medium optimization using factorial screening for docosahexaenoic acid production by Schizochytrium sp. sw1, American Journal of Applied Sciences 11(3), 462-474 (2014).
Original Article