Antimicrobial Activity Of Endophytic Fungi Isolated From Ocimum Sanctum Linn

  • Desale Monali G. Research Fellow, Dept. of Microbiology, Bharati Vidyapeeth University Yashwantrao Mohite College, Pune
  • Bodhankar M. G. Dean, Faculty of Science, Bharati Vidyapeeth University, Yashwantrao Mohite College, Pune
Keywords: Endophyte, Nigrospora oryzae, Ocimum sanctum, antimicrobial activity, bioactive compounds


The attention of scientific community has been drawn to the Endophytes due to their capacity to produce novel bioactive compounds. In the present study, the bioprospecting of fungal endophytes from Ocimum sanctum L. was studied and were screened for their antimicrobial potential. Use of different parts of the plant led to isolation of eighteen endophytic fungi.


Schulz, B., Boyle, C., Draeger, S., Rommert, A., and Krohn, K., , Endophytic fungi: a source of novel biologically active secondary metabolites, J. Mycological Society; 106, 996-1004 (2002)

Aly, A.H., Debbab,A., Kjer, J., Proksch, P., Fungal endophytes from higher plants: a prolific source of phytochemicals and other bioactive natural products. Fungal Diversity, 41,: 1-16 (2010)

Strobel, G., and Daisy, B., Bioprospecting for Microbial Endophytes and Their Natural Products. Microbiology and Molecular Biology Reviews,67(4),491-502 (2003)

Tan, R.X., Zou, W.X., , Endophytes: a rich source of functional metabolites,Nat Prod Rep,18, 448-59 (2001)

Bacon, C.W., and White, J.F., Physiological adaptations in the evolution of endophytism in Clavici pitaceae. In: Microbial Endophytes. Marcel Dekker, New York, pp,237 – 262 (2000)

Petrini, O., Fungal endophytes of tree leaves. In: Microbial ecology of leaves (eds. J Andrews, SS Hirano) New York, Springer, 179–197 (1991)

Strobel, G. A., Microbial gifts from rain forests. Can. J. Plant Pathology, 24,14-20 (2002)

Strobel, G. A., Dirksie, E., Sears, J. and Markworth, C. Volatile antimicrobialsfrom Muscodor albus, a novel endophytic fungus, Microbiology 147:2943–2950 (2001)

Suthep, W., Nongluksna, S., Wattana, P., Nuntawan, T., Kannawat, D., Nijsiri, R. and Vithaya, M., Endophytic fungi with antimicrobial, anti-cancer and anti-malarialactivities isolated from Thai medicinal plants, World J. Microb. Biotechnol, 20,265–272 (2004)

Stinson, M., Ezra, D., Hess, W.M., Sears, J., Strobel, G., An endophytic Gliocladium sp. of Eucryphia cordifolia producing selective volatile antimicrobial compounds, Plant Science, 165: 913-922 (2003)

Gangadevi, V., Muthumary, J., Preliminary studies on cytotoxic effect of fungal taxol on cancer cell lines, African Journal of Biotechnology, 6, 1382-1386 (2007)

Wang, J., Huang, Y., Fang, M., Zhang, Y., Zheng, Z., Zhao, Y., Su, W., Brefeldin, A., , A cytotoxin produced by Paecilomyces sp. and Aspergillus clavatus isolated from Taxus mairei and Torreya grandis. FEMS Immunol. Medical Microbiology,34,51-57 (2002)

Jayaweera, D.M., Medicinal Plants (Indigenous and Exotic) used in Ceylon, Part 2 A Publication of the Natural Sciences Council of Sri Lanka, Colombo.(1980)

Anonymous., Dictionary of Indian Medicinal Plants, Central Institute of Medicinal and Aromatic Plant, India.( 1992)

Perry, L.M., Medicinal Plants of East and South East Asia, Attributed properties and uses, MIT Press, London(1980)

Ocimum sanctum. The Indian home remedy. In:Current Medical Scene, March-April (Edited and published by S. Rajeshwari, Cipla Ltd., BombayCentral, Bombay (1992)

Pandey BP, Anita. In: Economic Botany (Published by Chand and Company Ltd., Ramnagar, New Delhi), p. 294, (1990)

Petrini, O., 1986, Taxonomy of endophytic fungi of aerial plant tissues. In Microbiology of the Phyllosphere (ed. N. j. Fokkema & van den Heuvel), pp. 175-187, Cambridge University Press, Cambridge.

Schulz, B., Wanke, U., Drager, S. and Aust, H.J., Endophytes from herbaceous plants and shrubs: effectiveness of surface sterilization methods. Mycological Research 97,1447-1450 (1993)

Chaoudhary, M. I., Musharraf, T., Shaheen, F., Ali, S., Atta-ur-rehman and Naturforsch, Z. C., Isolation of bioactive compounds from Aspergillus terreus.Journal of Bioscience,59:324-327 (2004)

Banerjee, D., Manna, S., Mahapatra, S. and Patil, B. R. . Fungal endophytes in three medicinal plants of Lamiaceae. Acta. Microbiol. Immunol. Hung. 56:243–50 (2009).
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