• Mahboob Ali Kalhoro Pharmaceutical Research Centre, PCSIR Labs Complex, Karachi
  • Sadia Farheen Department of Chemistry,University of Karachi
  • Noor-Ul-Aqsa Department of Chemistry,University of Karachi
Keywords: Vitex agnus castus, Antimicrobial activity, Chloramphenicol, Microrganisms


The ethanol extract of Vitex agnus castus was conducted to investigate the antimicrobial activity by agar well diffusion assay against Staphylococcus aureus, Streptococcuc intermedius, Bacillus cereus, Bacillus pumilus Echrichia coli, Proteus mirabilis and Candida albican. Antimicrobial activity was evaluated at 100, 50, 25and 10mg/ml concentration of ethanol extract.


Alam, M. I., Gomes, A., Snake venom neutralization by Indian medicinal plants (Vitex negundo and Emblica ofjicinalis) root extracts. J. Ethnopharmacol., 86, 75-80 (2003).

Anonymous, Compendium of medicinal plants used in Malaysia Vol. 2, Herbal Medicine Research Center, Institute for Medical Research, Kuala Lumpur, Malaysia, p. 417 (2002).

Avadhoot, Y., Rana, A. c., Hepatoprotective effect of Vitex negundo against carbon tetrachlorideinduced liver damage. Arch. Pharm. Res., 14, 96-98 (1991).

Baquar, S. R., Medicinal and poisonous plants of Pakistan, Printas Karachi, Pakistan, 472-473 (1989).

Chadha, Y. R., The Wealth ofIndia: A dictionary ofIndian raw material and industrial products, vol. 10, Publication Information Directorate, CSIR, New Delhi, pp. 522-524 (1976).

Chawla, A. S., Sharma, A. K., Handa, S. S., Dhar, K. L., Chemical investigation. and anti-inflammatory activity of Vitex negundo seeds. J. Nat. Prod., 55, 163-167 (1992).

Dharmasiri, M. G., Jayakody, 1. R., Ga]hena, G., Liyanage, S. S., Ratnasooriya, W. D., Antiinflammatory and analgesic activities of mature fresh leaves of Vitex negundo. J. Ethnopharmacol., 87, 199-206 (2003).

Gupta, M., Mazumder, U. K., Bhawal, S. R., CNS activity of Vitex negundo Linn. in mice. Indian Journal of Experimental Biology, 37,143-146 (1999).

I.Ahmed, Z. Mehmood and F.Mohammad, Journal of Ethnopharmacology.,62,183(1998).

Kirtikar, K.R., Basu, B.M., 1918. Indian Medicinal Plants, Apura Krishna Bose Indian Press, Allahabad, India, pp.2668-2672

Nadkami, K.M. 1976. Indian Materia Medica, Volume I (Revised and enlarged by Nadkarni, A.K.), Popular Prakashan Pvt. Ltd., Bombay, pp. 1278-1280.

Perry, L.M., 1980. Medicinal Plants of East and Southeast Asia. The M.LT Press U.S.A, pp. 430-431.

Prajapati, V., Tripathi, A.K., Khanuja, S.P.S., Kumar, S., 2003. Antisect screening of medicinal plants from Kukrail forest, Lukhnow, India. Pharmaceutical Biology 41, 166-170.

Tamhankar, c.P., Saraf, M.N., 1994. Anti-arthritic activity of V. negundo L. Indian Journal of Pharmaceutical Sciences 56, 552-553.

T.Sohail,Z.Yaqeen,H.Imran,Z.Rehman and N.Fatima Journal of Chemical Society Pakistan.,35,804 (2013)

World Health organization,GeneralGuidelines for Methodologies on Research and Evaluation of Traditional Medicine.WHO, Geneva, Switzerland.,p.1 (2001)
Original Article