Characteristic and cytotoxic activity of different α-Lactalbumin/fatty acids nanocomplex

  • Heba H. Salama Dairy Science Department, National Research Center, Cairo, Egypt
  • Mervat I. Foda Dairy Science Department, National Research Center, Cairo, Egypt
  • M. M. El-Sayed Dairy Science Department, National Research Center, Cairo, Egypt
  • Z.M.R Hassan Food Science Department,Faculty of Agriculture,Ain Shams University, Cairo, Egypt
  • R. A. Awad Food Science Department,Faculty of Agriculture,Ain Shams University, Cairo, Egypt
  • D. Otzen Interdisciplinary Nanoscience Center, Department of Molecular Biology, Faculty of Science,Aarhus University, Aarhus, Denmark
Keywords: Nanocomplex, α-lactalbumin, fatty acids, circulardichroism, cytotoxicity activity

Abstract

Theaim of this research wasto study the possibility of preparing a bovine α-lactalbumin/fatty acids nanocomplex with cytotoxic activity similar to the human alpha-lactalbumin made lethal to tumor cells (HAMLET). So, five different fatty acids with different concentrations were used to prepare nanocomplexes withbovine α -lactalbumin (α-LA) in ratio of 1mg/ml. Different measurements such as the Surface Tension, Circular Dichroism, Turbidity, and Cytotoxic activity for both individual fatty acid and α–LA/fatty acids nanocomplex were recorded.

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Published
2015-04-30
Section
Original Article