Statistical Modeling and Optimization of Algae Oil Extraction Using Response Surface Methodology

  • Kamen, F.L. Federal University of Technology, Owerri, Imo, Nigeria
  • Ejim I.F. Institute of Management and Technology, Enugu, Nigeria
  • Nwakaudu, M.S. Federal University of Technology, Owerri, Imo, Nigeria
  • Onyelucheya O.E. Federal University of Technology, Owerri, Imo, Nigeria
Keywords: Algae Oil, Box-Behnken Design, Extraction, Modeling, Response Surface Methodology


In this experiment, algae oil extraction with a combination of n-hexane and ethanol was investigated. The effects of extraction solvent concentration, extraction time and temperature on the yield and quality of oil were studied using Response Surface Methodology (RSM). Optimization of algae oil extraction using Box-Behnken design was used to generate 17 experimental runs in a three-factor-three-level design where oil yield, specific gravity, acid value and saponification value were evaluated as the response.


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